Anti-ATP2B1 Monoclonal Antibody for WB, IF/ICC, ELISA - P20020

Core Product Specifications and Parameters

Biological Background: ATP2B1 Function and Localization

• ATP2B1 (PMCA1) is a P-type calcium pump that couples ATP hydrolysis to calcium extrusion, crucial for maintaining resting cytoplasmic calcium levels. Related references: PMID:35358416
• PMCA1 regulates blood pressure by controlling intracellular calcium and nitric oxide production in vascular smooth muscle cells, affecting vasoconstriction.
• In bone, PMCA1 promotes mineralization by facilitating intestinal calcium absorption and dual roles in osteoclast differentiation/survival via calcium oscillations and extrusion.
• PMCA1 modulates insulin sensitivity through calcium/calmodulin-dependent activation of AKT1 and NOS3 in endothelial cells. Related references: PMID:29104511
• It may also contribute to synaptic transmission by regulating calcium and proton dynamics at presynaptic vesicles.
• The protein localizes to the plasma membrane, basolateral membrane, and synaptic vesicle membranes, reflecting diverse functional compartments.
• Multiple isoforms (B, C, D, K) exhibit tissue-specific expression: isoform B is ubiquitous, C in brain/heart/skeletal muscle, D fetal muscle, K intestine/liver and endometrial epithelium.
• ATP2B1 is subject to post-translational modifications including acetylation and phosphorylation, and contains domains for ATP binding, calcium transport, and calmodulin regulation.

Experimental Guidance and Technical Tips

• For western blotting, expect a specific band around 135 kDa under reducing conditions; consider using whole-cell lysates from cell lines expressing PMCA1.
• When performing immunofluorescence/immunocytochemistry, note the expected plasma membrane and vesicular staining patterns; permeabilization may be required for intracellular epitope detection.
• For ELISA applications, titrate the antibody in a standard sandwich or indirect format using a relevant recombinant protein or peptide to determine optimal working concentration.
• Validate the antibody in human, mouse, or rat samples, considering that the immunogen sequence (aa 5-103) is conserved across these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic-grade IVD raw materials, empowering researchers and manufacturers in calcium signaling, cardiovascular, and bone metabolism studies. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—ensuring seamless integration into your assay development pipeline. No matter the complexity of your project, CamelBio is your trusted partner from concept to clinic.