Anti-ATP23 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y6H3

Core Product Specifications and Parameters

Biological Background: ATP23 (Mitochondrial inner membrane protease ATP23 homolog) Function and Localization

• ATP23 is a mitochondrial inner membrane protease homolog, involved in processing proteins within the mitochondrial intermembrane space.
• The protein is also known as Ku70-binding protein 3 (KUB3) and XRCC6-binding protein 1 (XRCC6BP1), indicating potential associations with DNA repair proteins.
• Encoded by the ATP23 gene (aliases KUB3, XRCC6BP1), it belongs to the M16B family of metalloproteases and is classified under the reference proteome for humans.
• ATP23 functions as a metal-dependent hydrolase, requiring divalent metal ions for its protease activity.
• The canonical isoform has a predicted molecular weight of approximately 28 kDa, consistent with its identification in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (residues 1–246) of human ATP23; expect recognition of full-length protein and any N-terminal cleavage products.
• For western blot (WB), consider a starting antibody dilution based on typical polyclonal performance and validate the band pattern across human, mouse, and rat lysates.
• In immunohistochemistry (IHC‑P), optimize antigen retrieval and antibody concentration using tissue sections known to express ATP23 (e.g., mitochondria‑rich tissues).
• For immunofluorescence (IF/ICC), verify mitochondrial localization with a co‑staining marker to confirm specificity.
• When setting up ELISA, pair this antibody with an appropriate detection antibody and test against recombinant ATP23 or cell lysates to assess sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw‑material partner, providing validated antibody pairs, polyclonal and monoclonal antibodies, and ancillary reagents to support mitochondrial biology and protease research. Explore our catalog for the ATP23 rabbit pAb and complement it with matched detection reagents for robust assay development. For custom or bulk requirements, our sourcing team can rapidly connect you with high‑quality raw materials to accelerate your project from concept to clinic.