Anti-ATP1B3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P54709

Core Product Specifications and Parameters

Biological Background: ATP1B3 Function and Localization

• ATP1B3 encodes the sodium/potassium-transporting ATPase subunit beta-3, also known as ATPB-3 or CD298. It is a non-catalytic subunit of the Na+/K+-ATPase enzyme complex.
• The active enzyme catalyzes ATP hydrolysis, driving the exchange of Na+ and K+ ions across the plasma membrane, essential for maintaining electrochemical gradients.
• The exact function of the beta-3 subunit remains unclear; it may play a regulatory or structural role in the pump complex.
• ATP1B3 is localized to the apical and basolateral cell membranes, and has also been identified in melanosomes, indicating a potential role in melanosome physiology.
• The protein undergoes alternative splicing, producing isoforms that may differ in function or localization.
• Key post-translational modifications include disulfide bond formation and glycosylation, which contribute to the stability and function of the mature protein.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 180–279 of human ATP1B3. The antibody may detect both full-length and processed forms. Cross-reactivity with mouse and rat is documented, making it suitable for these model organisms.
• For Western blot, bands around 22 kDa and 32 kDa are typically observed, likely representing unglycosylated and glycosylated forms. Consider using reducing conditions and validate in your sample system.
• For immunohistochemistry (paraffin), antigen retrieval (e.g., heat-mediated citrate buffer) and optimization of primary antibody dilution are recommended. Include appropriate controls to confirm specificity.
• For ELISA, the antibody can be used as a detection or capture reagent; titrate against your specific antigen and check for cross-reactivity with other ATPase subunits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with premium IVD raw materials like this ATP1B3 rabbit monoclonal antibody. In addition to validated antibody pairs and monoclonal/polyclonal offerings, CamelBio provides bulk ancillary reagents and supports rare-target raw-material sourcing for ion transport and membrane protein research. Our streamlined procurement and technical consulting help accelerate your project from concept to clinic.