Anti-ATP1B2 Rabbit Polyclonal Antibody for WB - P14415

Core Product Specifications and Parameters

Biological Background: ATP1B2 Function and Localization

• ATP1B2 encodes the beta-2 subunit of the sodium/potassium-transporting ATPase, a non-catalytic component of the active enzyme complex responsible for Na⁺/K⁺ exchange across the plasma membrane.
• Beyond its role in ion transport, the beta-2 subunit (also known as AMOG) mediates cell adhesion between neurons and astrocytes and promotes neurite outgrowth, highlighting its dual function in neural tissues.
• This subunit is a type II membrane glycoprotein with a signal-anchor topology, localized primarily at the cell membrane.
• ATP1B2 contains conserved disulfide bonds that stabilize its extracellular domain, a feature critical for proper folding and adhesive interactions.
• The protein is expressed in glial and neural cells, consistent with its adhesion molecule in glia (AMOG) alias, and contributes to nervous system development and maintenance.
• UniProt keywords include Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, Ion transport, Potassium transport, Sodium transport, and Signal-anchor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 68–290 of human ATP1B2, covering a large extracellular portion; this region contains key structural motifs such as the disulfide-bonded domain, likely eliciting antibodies that recognize native conformations.
• When using this antibody for western blot (WB), expect a band at approximately 33 kDa under reducing conditions; glycosylation may cause slight mobility shifts in some tissues or cell lines.
• For immunofluorescence (IF/ICC), cell membrane localization should be observed; permeabilization is required as the epitope is extracellular but the antibody is not directly labeled.
• The reported cross-reactivity with mouse and rat allows for translational studies across species; however, validation in the relevant sample system is recommended to confirm reactivity and specificity.
• This polyclonal antibody is suitable for ELISA-based detection; consider optimizing coating and detection conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This ATP1B2 polyclonal antibody exemplifies our catalog of membrane transporter and adhesion molecule tools, supporting neurological and biomarker research from concept to clinic. Leverage our integrated pipeline to streamline your procurement and accelerate diagnostic development.