Anti-ATP1B2 Polyclonal Antibody for IHC-P, ELISA - P14415

Core Product Specifications and Parameters

Biological Background: ATP1B2 Function and Localization

• ATP1B2 is the beta-2 subunit of the Na+/K+-ATPase, a non-catalytic component of the active enzyme that hydrolyzes ATP coupled with Na(+) and K(+) exchange across the plasma membrane.
• The protein mediates cell adhesion of neurons and astrocytes and promotes neurite outgrowth, highlighting its role in neural development and function.
• It is also known as Adhesion molecule in glia (AMOG), reflecting its dual role in ion transport and cell adhesion.
• ATP1B2 is localized at the cell membrane, anchored via a signal-anchor transmembrane domain.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for its stability and function.
• Keywords from UniProt include: Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, and Transport.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 68-290, a region likely containing extracellular domains, making the antibody suitable for detecting cell surface ATP1B2 in IHC-P. Validate antigen retrieval conditions in tissue sections known to express ATP1B2, such as brain.
• For ELISA, the antibody can be used to capture or detect recombinant or native ATP1B2. Optimize coating concentrations and blocking steps for best signal-to-noise ratios.
• As a polyclonal antibody, lot-to-lot consistency may vary; consider testing each new lot against a standard reference and using internal controls.
• The antibody cross-reacts with mouse and rat, allowing multi-species experimental designs. Confirm reactivity in your specific model system.
• Perform a dilution series (e.g., 1:100–1:1000 for IHC-P) to determine the optimal working concentration for your assay.

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CamelBio provides diagnostic manufacturers and laboratories with a comprehensive range of IVD raw materials, including validated antibodies like the ATP1B2 polyclonal antibody for ion transport and neuroscience research. Our procurement capabilities extend to antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, ensuring you have reliable access to rare and routine targets from concept to clinic.