Anti-ATP1B1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P05026

Core Product Specifications and Parameters

Biological Background: ATP1B1 Function and Localization

• ATP1B1 encodes the beta-1 subunit of the Na+/K+-ATPase, a non-catalytic component that assembles with the catalytic alpha subunit to regulate sodium pump density at the plasma membrane. Related references: PMID:19694409
• It is essential for cell adhesion and establishment of epithelial cell polarity.
• The protein is expressed in most tissues.
• Subcellularly, it localizes to the cell membrane, including apical membranes and sarcolemma.
• ATP1B1 is involved in innate immunity by enhancing virus-triggered induction of interferons and ISGs through modulation of TRAF3/6 ubiquitination and TAK1/TBK1 phosphorylation. Related references: PMID:34011520
• Possesses post-translational modifications including glycosylation, disulfide bonds, glutathionylation, and phosphorylation.
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the cytoplasmic domain of ATP1B1; the antibody may recognize both unmodified and post-translationally modified forms.
• For WB, the predicted molecular weight is ~35 kDa, though glycosylated forms may migrate higher; validate in the relevant sample system.
• Consider testing the antibody in WB, IHC-P, and ELISA using extracts or tissue sections from human, mouse, or rat, as cross-reactivity is indicated.
• Optimize working dilutions for each application by titration experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-ATP1B1 reagent for ion transport, cell adhesion, and innate immunity studies. We also supply bulk ancillary reagents and specialize in sourcing rare-target raw materials, ensuring a seamless procurement experience from concept to clinic.