Anti-ATP1A4 Polyclonal Antibody for WB and ELISA - Q13733

Core Product Specifications and Parameters

Biological Background: ATP1A4 Function and Localization

• ATP1A4 encodes the sperm-specific alpha-4 catalytic subunit of the Na+/K+-ATPase, a transmembrane ion pump that couples ATP hydrolysis to the exchange of 3 Na+ out and 2 K+ into the cell. Related references: PMID:16861705
• This ATP1A4 isoform is essential for sperm motility, capacitation, and male fertility by maintaining ionic gradients required for flagellar function and cellular signaling (By similarity).
• The protein is an integral component of the cell membrane, consistent with its role as a P-type ATPase transporter, and contains at least one transmembrane helix and an ATP-binding domain (Keywords).
• ATP1A4 expression is highly restricted to the testis, with the protein detected at high levels in mature sperm and only trace mRNA found in skeletal muscle. Related references: PMID:16175638, PMID:16861705
• Post-translational modifications include phosphorylation, and the protein requires Mg2+ for its enzymatic activity, binding ATP and the transported cations Na+ and K+ (Keywords).
• Alternative splicing of ATP1A4 produces multiple isoforms, which may exhibit tissue-specific or developmental regulation (Keywords).
• The ATP1A4 gene belongs to the ATP1A family of sodium/potassium ATPase alpha subunits, which also includes ubiquitously expressed forms such as ATP1A1.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1‑90) of human ATP1A4, suggesting the antibody may preferentially recognize the N‑terminus of the full‑length protein; consider this when designing epitope‑blocking or competition assays.
• For Western blotting, a band at approximately 114 kDa is expected in samples from testis or mature sperm; validate the antibody in the relevant sample system (e.g., human, mouse, or rat testis lysates) due to the reported cross‑reactivity.
• When using ELISA, the antibody can be employed for antigen capture or detection; perform standard checkerboard titrations to determine optimal working concentrations for your specific assay format.
• Given the restricted expression of ATP1A4, ensure that negative control samples (e.g., non‑testis tissue or cell lines) are included to confirm specificity.
• The antibody is supplied as a rabbit polyclonal, which may exhibit lot‑to‑lot variability; it is advisable to reserve a reference lot for long‑term reproducibility studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutions with high-quality reagents and technical support. This anti-ATP1A4 polyclonal antibody supports research into sperm biology and fertility, and CamelBio can also supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.