Anti-ATP1A4 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13733

Core Product Specifications and Parameters

Biological Background: ATP1A4 Function and Localization

• ATP1A4 encodes the sperm-specific alpha-4 subunit of the Na+/K+-ATPase, a P-type ATPase that actively transports Na+ and K+ across the plasma membrane.
  Related references: PMID:16861705
• This subunit is a catalytic component essential for ATP hydrolysis and ion translocation, coupling the transport of three Na+ ions out and two K+ ions into the cell per ATP molecule.
• ATP1A4 is critical for sperm motility, capacitation, and male fertility, as it maintains the ionic gradients necessary for flagellar movement and signaling.
• The protein is localized to the cell membrane.
• Expression is highly specific to the testis and mature sperm, with very low levels detected in skeletal muscle.
  Related references: PMID:16175638, PMID:16861705
• Post-translational modifications, including phosphorylation, regulate the activity and kinetics of the pump.
• Alternative splicing of ATP1A4 generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 401-530 within the cytoplasmic region of human ATP1A4, a domain involved in ATP binding and hydrolysis; consider this when interpreting results from functional assays.
• For Western blotting, the antibody is expected to recognize the full-length ATP1A4 protein at around 114 kDa, with a possible fragment at 19 kDa; validate in your specific sample system.
• Due to the testis-specific expression, suitable positive controls for IHC and IF include human testis tissue or sperm samples; confirm expression in your experimental model.
• For ELISA applications, pair with an appropriate capture antibody or antigen to ensure specificity; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, from validated antibody pairs to optimized monoclonal and polyclonal antibodies. This anti-ATP1A4 antibody supports studies in reproductive biology and male fertility, and CamelBio can source additional targets, bulk reagents, and rare raw materials to streamline your development pipeline.