Anti-ATP1A2 Monoclonal Antibody for WB, ELISA - P50993

Core Product Specifications and Parameters

Biological Background: Sodium/potassium-transporting ATPase Subunit Alpha-2 Function and Localization

• ATP1A2 encodes the alpha-2 subunit of the Na+/K+-ATPase, an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane.
• The enzyme hydrolyzes one ATP molecule per transport cycle, driving the export of three Na+ ions out of the cell and import of two K+ ions into the cell, an essential process for cellular excitability and volume regulation. Related references: PMID:33880529
• As a member of the P-type ATPase family, ATP1A2 undergoes phosphorylation during the catalytic cycle and contains conserved binding sites for ATP, magnesium, and the transported cations.
• ATP1A2 is a multi-pass transmembrane protein localized primarily to the cell membrane, where it functions as a critical ion pump in many tissues, including brain, heart, and muscle.
• Mutations in ATP1A2 are associated with familial hemiplegic migraine type 2 (FHM2) and other neurological disorders, highlighting its role in neuronal function and ion homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1-100) of human ATP1A2; consider this when designing controls or epitope competition assays.
• For Western blotting, the predicted molecular weight is approximately 112 kDa; optimize reducing conditions and electrophoresis protocols for high‑molecular‑weight membrane proteins.
• In ELISA applications, validate the coating antigen and antibody concentration in the relevant sample matrix to ensure specificity.
• Since this is a rabbit monoclonal antibody, use an appropriate anti‑rabbit secondary antibody and run species‑matched controls when working with mouse or rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for in vitro diagnostic (IVD) raw material procurement, connecting diagnostic manufacturers, labs, and research institutes with high‑quality antibodies and reagents. This anti‑ATP1A2 monoclonal antibody supports research into ion transport, neurological disease mechanisms, and cell signaling. Beyond individual antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare‑target raw materials to streamline your diagnostic development and research.