Anti-ATP13A2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQ11

Core Product Specifications and Parameters

Biological Background: ATP13A2 Function and Localization

• ATP13A2 (polyamine-transporting ATPase 13A2, also known as PARK9, CLN12, KRPPD, SPG78) functions as a lysosomal polyamine exporter with high affinity for spermine, protecting cells from polyamine toxicity and stimulating polyamine uptake.
  Related references: PMID:31996848
• Plays a key role in intracellular cation homeostasis, zinc homeostasis, and protection against manganese and zinc toxicity, thereby maintaining neuronal integrity.
  Related references: PMID:22186024, PMID:24603074, PMID:26134396
• Required for proper lysosomal and mitochondrial maintenance, and regulates the autophagy-lysosome pathway through transcriptional and post-translational control of SYT11, as well as HDAC6 recruitment to promote autophagosome-lysosome fusion.
  Related references: PMID:22296644, PMID:28137957, PMID:27278822, PMID:30538141
• Promotes secretion of exosomes, including SCNA, and plays a role in lipid homeostasis.
  Related references: PMID:24603074, PMID:25392495, PMID:31132336
• Localizes to lysosome membrane, late endosomes, multivesicular bodies, and autophagosome membranes.
• Highly expressed in brain, particularly in pyramidal neurons of the cingulate cortex and dopaminergic neurons of substantia nigra; protein levels are increased in Parkinson disease and dementia with Lewy bodies.
• Mutations in ATP13A2 cause neurodegenerative disorders including Kufor-Rakeb syndrome (PARK9), neuronal ceroid lipofuscinosis (CLN12), and hereditary spastic paraplegia (SPG78).
• Undergoes glycosylation and phosphorylation, as indicated by its annotation as a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70–210 of human ATP13A2, an N-terminal cytoplasmic region that is conserved across human, mouse, and rat, supporting cross-reactivity predictions.
• For Western blotting, expect a band at approximately 129 kDa; validate with appropriate controls in your sample system.
• In immunofluorescence/ICC, consider permeabilization and co-staining with lysosomal markers to confirm localization.
• ELISA applications may require optimization of coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti-ATP13A2 antibody supports research into Parkinson's disease, lysosomal storage disorders, and cellular polyamine homeostasis. With a global supply chain, we can source rare-target reagents and offer technical consulting to accelerate your diagnostic development.