Anti-ATP12A Polyclonal Antibody for WB, ELISA - P54707

Core Product Specifications and Parameters

Biological Background: Potassium-transporting ATPase alpha chain 2 (ATP12A) Function and Localization

• ATP12A encodes the catalytic alpha subunit of a non-gastric H+/K+ ATPase and/or Na+/K+ ATPase, also known as HK alpha 2, proton pump, or sodium pump. Related references: PMID:11341842, PMID:7485470, PMID:8853415
• The pump couples ATP hydrolysis to the exchange of extracellular K+ for intracellular Na+ and/or H+ across the apical membrane, maintaining electrolyte homeostasis in kidney and colon. Related references: PMID:11341842, PMID:7485470, PMID:8853415
• In airway epithelium, ATP12A-mediated proton secretion acidifies airway surface liquid, regulating mucus viscosity and mucociliary clearance. Related references: PMID:29391451
• The protein localizes to the apical cell membrane of polarized epithelial cells.
• Expression is detected in airway epithelial cells, skin, kidney, and prostate basal cells, with upregulation in benign prostate hyperplasia and tumor tissues.
• ATP12A belongs to the P-type ATPase family and harbors domains for ATP binding, phosphorylation, and cation translocation, with a predicted molecular weight of ~115-116 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 890-980), which may be exposed in the native structure, supporting applications such as ELISA and denaturing Western blot.
• For Western blot, expect a band at approximately 116 kDa in human samples expressing ATP12A. Consider using appropriate positive controls (e.g., airway epithelial cell lysates).
• ELISA can be employed for titer determination and relative quantification; optimize coating conditions and antibody dilutions empirically.
• Cross-reactivity with species other than human has not been determined; validate in the intended sample system if non-human studies are planned.
• As a polyclonal antibody, note potential lot-to-lot variability; incorporate routine quality controls and consistent protocol parameters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes with a comprehensive one-stop channel for IVD raw materials—from concept to clinic. This anti-ATP12A polyclonal antibody supports investigations into ion transport, mucus biology, and epithelial disorders, with applications in Western blot and ELISA. Beyond validated antibodies, CamelBio supplies bulk ancillary reagents and tackles rare-target raw-material sourcing, ensuring seamless progression from assay development to scalable production.