Anti-ATP10A Polyclonal Antibody for WB and ELISA - O60312

Core Product Specifications and Parameters

Biological Background: ATP10A Function and Localization

• ATP10A is the catalytic subunit of a P4-ATPase flippase complex that couples ATP hydrolysis to the inward transport of phosphatidylcholine (PC) at the plasma membrane, establishing phospholipid asymmetry. Related references: PMID:25947375, PMID:29599178, PMID:30530492
• By initiating inward plasma membrane bending and recruiting BAR domain-containing proteins, ATP10A plays a role in membrane tubulation and intracellular trafficking. Related references: PMID:29599178
• ATP10A facilitates the endocytosis of ITGB1/beta1 integrin, thereby delaying cell adhesion and spreading on the extracellular matrix. Related references: PMID:25947375, PMID:29599178
• ATP10A shows low flippase activity toward glucosylceramide (GlcCer), suggesting a role in lipid transport beyond phospholipids. Related references: PMID:30530492
• The protein localizes to the cell membrane and endoplasmic reticulum membrane, consistent with its function in membrane dynamics and lipid transport.
• ATP10A is widely expressed, with the highest levels detected in kidney, lung, brain, prostate, testis, ovary, and small intestine.
• It belongs to the P-type ATPase family and contains conserved motifs for ATP binding, magnesium binding, and phosphorylation, as well as multiple transmembrane helices.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 440-690 of human ATP10A, covering a cytoplasmic domain that may be suitable for recognizing both the full-length and potential endogenous forms.
• This polyclonal antibody is validated for Western blot (WB) and ELISA applications; for WB, consider a starting dilution range guided by the target's predicted molecular weight of ~168 kDa.
• The predicted molecular weight of 168 kDa is based on the theoretical sequence; actual migration may vary, and post-translational modifications could result in additional bands above the predicted size.
• Cross-reactivity is reported for human; validation in other species is not confirmed and should be tested if required.
• For optimal performance, validate in the relevant sample system, such as human kidney or lung tissues/lines that express high ATP10A levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to validated IVD raw materials such as this ATP10A polyclonal antibody, suited for studies of membrane lipid asymmetry and cell adhesion. Beyond single reagents, we offer matched antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your entire assay development pipeline.