Anti-ATOH8 Polyclonal Antibody for WB and ELISA - Q96SQ7

Core Product Specifications and Parameters

Biological Background: ATOH8 Function and Localization

• ATOH8 is a basic helix-loop-helix (bHLH) transcription factor that binds to canonical E-box sequences (5'-CANNTG-3'), likely as a heterodimer with other bHLH proteins. Related references: PMID:24236640

• It regulates endothelial cell proliferation, migration, and tube formation, and modulates endothelial cell differentiation through NOS3. Related references: PMID:24463812

• It positively regulates hepcidin (HAMP) transcription by direct promoter binding and indirect SMAD phosphorylation. Related references: PMID:24236640

• May participate in neuronal cell lineage specification, kidney development, podocyte differentiation, early embryonic tissue differentiation, and myogenesis (by similarity).

• Represses NEUROG3-dependent gene activation by heterodimerizing with TCF3 and DNA binding (by similarity).

• Localizes to the nucleus, nuclear speckles, and cytoplasm; alternative splicing produces multiple isoforms.

• Expressed in lung, liver, kidney, heart, pancreas, and endothelial cells of umbilical vessels.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 150–250 of human ATOH8. This polyclonal antibody is suitable for western blotting (WB) and ELISA applications.
• For WB, the predicted molecular weight of ATOH8 is approximately 35 kDa. Bands near this size may be observed; validate in relevant cell or tissue lysates.
• Cross-reactivity with mouse and rat is reported; consider validating in these species for your experimental system.
• When using in ELISA, the antibody can be employed as a capture or detection reagent; titrate and optimize conditions for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, providing high-quality antibodies, antigen pairs, and ancillary reagents for transcription factor and developmental biology research. Our ATOH8 polyclonal antibody enables reliable detection in western blot and ELISA, supporting your assay development from concept to clinic. Contact us for bulk orders, customized solutions, and technical support for bHLH transcription factor-related projects.