Anti-ATOH8 Polyclonal Antibody for WB and ELISA - Q96SQ7

Core Product Specifications and Parameters

Biological Background: ATOH8 Function and Localization

• ATOH8 is a basic helix-loop-helix (bHLH) transcription factor, also referred to as bHLHa21 or hATH6.
• It binds to the canonical E-box DNA sequence 5'-CANNTG-3', possibly as a heterodimer with other bHLH proteins, to regulate gene transcription. Related references: PMID:24236640
• ATOH8 regulates endothelial cell proliferation, migration, and tube-like structure formation, and modulates endothelial cell differentiation through NOS3. Related references: PMID:24463812
• It may participate in neuronal cell lineage specification in the brain, kidney development, and podocyte differentiation, and modulates NEUROG3-initiated differentiation programs.
• During myogenesis, ATOH8 may play a role in the transition from myoblast proliferation to differentiation.
• It positively regulates HAMP transcription by direct promoter binding and by enhancing SMAD phosphorylation. Related references: PMID:24236640
• ATOH8 represses NEUROG3-dependent gene activation through heterodimerization with general partners and potentially through direct DNA binding.
• Subcellular localization includes the nucleus (particularly nuclear speckles) and cytoplasm.
• The protein is expressed in lung, liver, kidney, heart, pancreas, and endothelial cells of umbilical vessels.
• Key functional annotations include alternative splicing, developmental protein, differentiation, DNA-binding, neurogenesis, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N‑terminal region (amino acids 1–100). For Western blotting, consider optimizing blocking and washing conditions to reduce non‑specific binding typical of polyclonal antibodies.
• In ELISA applications, use appropriate coating buffers and validate the antibody against the specific peptide or recombinant protein to ensure reactivity.
• The predicted molecular weight is 35 kDa, but actual migration in SDS‑PAGE may vary due to post‑translational modifications; always run appropriate molecular weight markers and positive controls.
• For indirect detection, choose a secondary antibody that matches the rabbit host and is validated for your detection method, and include proper control experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including this anti‑ATOH8 polyclonal antibody, supporting research into endothelial biology, development, and transcriptional regulation. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with rare‑target raw‑material sourcing to streamline your diagnostic and research workflows.