Anti-ATOH7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N100

Core Product Specifications and Parameters

Biological Background: ATOH7 Function and Localization

• ATOH7 (Math5, bHLHa13) is a basic helix-loop-helix (bHLH) transcription factor belonging to the atonal family, primarily implicated in neurogenesis and sensory neuron development.
• It binds to the consensus DNA sequence 5'-CAG[GC]TG-3' and may dimerize with the E-protein TCF3 (isoform E47) to regulate target gene expression. Related references: PMID:31696227
• ATOH7 governs a transcriptional program essential for retinal ganglion cell (RGC) determination and survival, photoreceptor population development, and proper targeting of RGC axons to the optic nerve and retino-hypothalamic tract.
• The protein can bind its own promoter and enhancer elements, suggesting an autoregulatory loop that fine-tunes its expression during retinal development.
• Beyond the retina, ATOH7 is required for retinal circadian rhythm photoentrainment and contributes to brainstem auditory signaling and binaural processing.
• Subcellularly, ATOH7 localizes predominantly to the nucleus, but is also detected in the perikaryon and axon projections, reflecting its regulatory and possible transport roles.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 47–152 of human ATOH7, a region that does not include the bHLH domain; verify that the antibody recognizes your target isoform under native and denaturing conditions.
• In western blotting, expect a principal band near 17 kDa; additional bands may arise from post-translational modifications or protein interactions—validate with appropriate controls.
• For immunofluorescence/immunocytochemistry, anticipate nuclear staining as well as possible perikaryal and axonal signals; optimize fixation and permeabilization for your cell type.
• When using ELISA, titrate both coating antigen and antibody concentrations to establish a linear detection range for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a complete portfolio of IVD raw materials, including well-characterized monoclonal and polyclonal antibodies, validated antibody pairs, and ancillary reagents. This anti-ATOH7 polyclonal antibody exemplifies our capability to supply high-quality transcription factor and neurogenesis tools for assay development and basic research. For bulk orders, custom antibody development, or technical consultation, please reach out to explore how CamelBio can accelerate your project from concept to clinic.