Anti-ATOH1 Polyclonal Antibody for WB, ELISA - Q92858

Core Product Specifications and Parameters

Biological Background: ATOH1 Function and Localization

• Transcription factor ATOH1 (also known as Class A basic helix-loop-helix protein 14, bHLHa14; helix-loop-helix protein hATH-1; protein atonal homolog 1) is encoded by the ATOH1 gene (Gene ID: 474).
• It functions as a transcriptional regulator that activates E box-dependent transcription, likely in collaboration with TCF3/E47, playing a role in the differentiation of subsets of neural cells.
• The transcriptional activity of ATOH1 is completely antagonized by the negative regulator of neurogenesis HES1.
• ATOH1 localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• It is classified as a developmental protein involved in differentiation and neurogenesis, and mutations are associated with non-syndromic deafness.
• The protein has a calculated molecular weight of 38,160 Da, migrating at approximately 38 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 215-354) corresponds to a region that may be accessible in endogenous ATOH1; consider validating the antibody in your specific sample system.
• For Western blotting, expect a band around 38 kDa based on the predicted molecular weight; include appropriate positive and negative controls.
• The antibody is suitable for ELISA; optimization of coating concentration and detection conditions is recommended.
• Given the nuclear localization, nuclear extracts may improve detection sensitivity in Western blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to validated IVD raw materials, including optimized polyclonal and monoclonal antibodies targeting key transcription factors like ATOH1. Our portfolio supports neurogenesis and hearing loss research with reliable reagents, bulk ancillary supplies, and rare target sourcing. Partner with us to streamline your assay development from concept to clinic.