Anti-ATOH1 Monoclonal Antibody for WB, ELISA - Q92858

Core Product Specifications and Parameters

Biological Background: ATOH1 Function and Localization

• Transcription factor ATOH1 (also known as MATH-1, HATH1, bHLHa14) is a basic helix-loop-helix (bHLH) transcription factor encoded by the ATOH1 gene; it belongs to the atonal family of proneural bHLH proteins.
• ATOH1 functions as a transcriptional regulator that activates E box-dependent transcription in collaboration with TCF3/E47; this activity is completely antagonized by the negative regulator of neurogenesis HES1 (By similarity).
• It plays a critical role in the differentiation of subsets of neural cells by activating E box-dependent transcription, contributing to neurogenesis and developmental processes (By similarity).
• Subcellularly, ATOH1 localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• The protein is associated with keywords such as activator, deafness, developmental protein, differentiation, DNA-binding, neurogenesis, non-syndromic deafness, nucleus, and transcription regulation; mutations in ATOH1 have been linked to non-syndromic deafness (DFNA89).
• The molecular weight of the canonical isoform is approximately 38.16 kDa, consistent with the observed protein weight of 38 kDa for this antibody target.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1–100) of human ATOH1; this region includes the bHLH domain critical for DNA binding and dimerization.
• For Western blotting, a band at approximately 38 kDa is expected; consider using nuclear extracts or whole-cell lysates from tissues or cell lines that express ATOH1, such as neural progenitors or hair cells.
• For ELISA, the antibody may be used as either capture or detection reagent; validate performance with recombinant ATOH1 protein or peptide in the relevant assay format.
• Cross-reactivity is confirmed for human; if needed for other species, evaluate sequence homology and consider performing species-specific validation.
• Because ATOH1 expression is often transient and cell-type specific, include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio bridges the gap between discovery and diagnostic development by supplying high-quality antibodies, proteins, and ancillary reagents. This ATOH1 monoclonal antibody supports research into neurogenesis, hearing loss, and developmental biology, enabling robust immunoassays. Whatever your target or scale, CamelBio offers validated antibody pairs, optimized monoclonals, and tailored sourcing solutions to streamline your diagnostic pipeline.