Anti-ATN1 Polyclonal Antibody for WB, IF/ICC, ELISA - P54259

Core Product Specifications and Parameters

Biological Background: ATN1 Function and Localization

• Atrophin-1 (ATN1), also known as dentatorubral-pallidoluysian atrophy protein, is a transcriptional corepressor involved in regulating gene expression.
• It recruits NR2E1 to repress transcription and functions as a corepressor for MTG8, with intrinsic repression activity independent of poly-glutamine repeat length.
• ATN1 promotes vascular smooth muscle cell migration and orientation (by similarity), suggesting a role in cardiovascular biology.
• The protein localizes to the nucleus and cytoplasm, particularly the perinuclear region, and is also found at cell junctions.
• ATN1 is widely expressed in adult tissues, with high levels in the brain, including the amygdala, caudate nucleus, and hippocampus, and elevated expression in fetal tissues.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.
• Mutations involving poly-glutamine tract expansion cause dentatorubral-pallidoluysian atrophy (DRPLA), a neurodegenerative disorder characterized by epilepsy, intellectual disability, and ataxia.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 900-1040) of human ATN1, which is distal to the polyQ repeat tract; this design may help reduce interference from repeat length polymorphisms in detection.
• For Western blot, the predicted molecular weight is approximately 125 kDa; ensure adequate transfer conditions for high-molecular-weight proteins.
• For immunofluorescence, consider permeabilization protocols that allow detection of both nuclear and cytoplasmic pools.
• Validate antibody specificity in the relevant human tissue or cell line model, especially those with known ATN1 expression, such as brain or fetal tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. This anti-ATN1 polyclonal antibody supports neuroscience and neurodegeneration research, particularly studies on DRPLA and transcriptional regulation. In addition to validated antibodies, CamelBio offers bulk ancillary reagents, custom antibody development, and rare-target sourcing services to accelerate your diagnostic development from concept to clinic.