Anti-ATM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13315

Core Product Specifications and Parameters

Biological Background: ATM Function and Localization

• ATM, or Ataxia telangiectasia mutated, is a serine/threonine protein kinase that acts as a DNA damage sensor, recognizing double-strand breaks (DSBs) and activating checkpoint signaling in response to genotoxic stresses such as ionizing radiation and ultraviolet A light (PubMed:10550055, PubMed:10839545, PubMed:10910365). Related references: PMID:10550055 PMID:10839545 PMID:10910365
• The kinase phosphorylates downstream effectors containing the consensus motif [ST]-Q, including histone H2AX at Ser-139, p53/TP53, CHEK2, BRCA1, MRE11, and many others, thereby coordinating DNA repair, cell cycle arrest, and apoptosis (PubMed:10550055, PubMed:10766245, PubMed:10802669). Related references: PMID:10550055 PMID:10766245 PMID:10802669
• ATM plays a key role in cell cycle control and is considered a tumor suppressor, with mutations in the ATM gene linked to ataxia-telangiectasia, a syndrome featuring neurodegeneration, immunodeficiency, and cancer predisposition.
• Subcellularly, ATM is primarily nuclear but also localizes to cytoplasmic vesicles, the centrosome, and peroxisomes. In response to reactive oxygen species (ROS), it translocates to peroxisomes and phosphorylates PEX5 to induce pexophagy (PubMed:26344566). Related references: PMID:26344566
• ATM is widely expressed in tissues including pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon, and leukocytes.
• The protein undergoes phosphorylation on multiple residues upon DNA damage, which is essential for its activation and subsequent substrate targeting.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 2320–2566 in the C-terminal region of ATM. This antibody is expected to recognize the full-length protein (351 kDa) and may react with isoforms containing this epitope; validate in your relevant sample system.
• For Western blotting, use appropriate gel percentage and transfer conditions (e.g., wet transfer with extended time) to efficiently blot this high-molecular-weight protein.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization to detect ATM in nuclear and cytoplasmic compartments; consider using known DNA damage-inducing treatments to evaluate localization changes.
• For ELISA, employ a reliable coating buffer and validate the antibody pair compatibility if used in a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a seamless one-stop sourcing platform for IVD raw materials, covering every stage from concept to clinic. We provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for DNA damage and cell cycle research. With our extensive supply network, we can also source rare-target raw materials and offer technical consulting to accelerate your assay development for cancer diagnostics and beyond.