Anti-ATM Rabbit Polyclonal Antibody for WB, ELISA - Q13315

Core Product Specifications and Parameters

Biological Background: ATM Function and Localization

• Serine/threonine protein kinase that activates checkpoint signaling upon DNA double-strand breaks (DSBs), apoptosis and genotoxic stresses, acting as a critical DNA damage sensor. Related references: PMID:10550055, PMID:10839545, PMID:10910365

• Recognizes the substrate consensus sequence [ST]-Q and phosphorylates key DNA damage response proteins including CHEK2, p53/TP53, BRCA1, and nibrin (NBN). Related references: PMID:10550055, PMID:10839545, PMID:10910365, PMID:10766245, PMID:10802669

• Phosphorylates histone variant H2AX at Ser-139 at double-strand breaks, thereby regulating the DNA damage response mechanism (By similarity).

• Phosphorylates diverse substrates including DYRK2, ATF2, ERCC6, TTC5/STRAP, and PEX5 to coordinate DNA repair, apoptosis, and cellular homeostasis. Related references: PMID:19965871, PMID:15916964, PMID:29203878, PMID:15448695, PMID:26344566

• Found in the nucleus, cytoplasmic vesicles, centrosomes, and peroxisome matrix, consistent with its roles in DNA damage signaling, transport, and pexophagy.

• Ubiquitously expressed, with highest levels in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Experimental Guidance and Technical Tips

• For western blot analysis, expect a band around 351 kDa. Due to the large protein size, consider using low‑percentage gradient gels, extended wet transfer times, and verify transfer efficiency with a pre‑stained high‑molecular‑weight marker.
• The immunogen is a synthetic peptide from the C‑terminal region (aa 1900–2000). Epitope accessibility may be influenced by protein conformation; denaturing and reducing conditions are recommended.
• As a polyclonal antibody, it may exhibit lot‑to‑lot variability. Validate each new lot on a known positive control (e.g., human cell lysate expressing ATM) before use.
• For ELISA, optimize coating antigen concentration and detection conditions. The antibody is predicted to recognize human ATM; cross‑reactivity with other species has not been tested.
• To confirm specificity, consider using ATM‑depleted samples (e.g., siRNA knockdown) or a competing peptide in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one‑stop sourcing partner, offering diagnostic manufacturers and research laboratories seamless access to premium IVD raw materials—from validated antibody pairs to bulk ancillary reagents and rare target sourcing. This ATM antibody supports oncology and DNA damage response research, and our team can help you accelerate development from concept to clinic.