Anti-ATM Monoclonal Antibody for WB, IHC-P, ELISA - Q13315

Core Product Specifications and Parameters

Biological Background: ATM Function and Localization

• ATM (Ataxia telangiectasia mutated) is a serine/threonine protein kinase encoded by the ATM gene, belonging to the PIKK family of kinases involved in DNA damage signaling.
• Functions as a primary sensor of DNA double-strand breaks (DSBs) and genotoxic stress, such as ionizing radiation and ultraviolet A light. It recognizes and phosphorylates substrate proteins on the consensus motif [ST]-Q to initiate checkpoint signaling, apoptosis, and DNA repair. Related references: PMID:10550055, PMID:10839545, PMID:10910365
• Phosphorylates numerous downstream effectors, including histone H2AX (at Ser-139), CHEK2, p53/TP53, BRCA1, and FANCD2, thereby coordinating cell cycle arrest and DNA damage repair pathways. Related references: PMID:10550055, PMID:10766245, PMID:10802669
• Localizes to the nucleus, where it primarily mediates DNA damage signaling, but also resides in cytoplasmic vesicles, centrosomes, and peroxisomes, reflecting its diverse cellular roles.
• Widely expressed in human tissues, with detection in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon, and leukocytes.
• Regulates pexophagy by phosphorylating PEX5 at peroxisomes in response to reactive oxygen species, targeting it for ubiquitination and degradation. Related references: PMID:26344566
• Additional functions include enforcing allelic exclusion during B-cell development, controlling replication-dependent histone mRNA degradation, and acting as a potential tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2619–2939 of human ATM, located in the C-terminal region. Validate antibody specificity using appropriate controls, especially when targeting isoforms or splice variants.
• For Western blot, ATM migrates at approximately 351 kDa. Use low-percentage polyacrylamide gels (e.g., 6–8%) and extended transfer times to ensure efficient transfer of this large protein.
• In IHC-P, optimize antigen retrieval methods (e.g., heat-induced epitope retrieval) and primary antibody concentration. Consider using tissues with high ATM expression, such as testis or pancreas, as positive controls.
• Cross-reactivity with mouse is reported; if using mouse models, confirm suitability by testing in relevant knockout or knockdown systems.
• As a monoclonal antibody, it offers high specificity. Include both positive and negative biological controls (e.g., ATM-proficient and ATM-deficient cell lines) to verify band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, from validated monoclonal antibodies like this anti-ATM clone to complementary reagents and custom development services. Focused on DNA damage and cancer research areas, we supply optimized antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate diagnostic assay development.