Anti-ATL2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NHH9

Core Product Specifications and Parameters

Biological Background: ATL2 Function and Localization

• ATL2, also known as ARL6IP2 or atlastin-2, is a member of the atlastin family of GTPases with a molecular weight of approximately 66 kDa.
• Mediates GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network, and facilitates the formation of three-way junctions where ER tubules intersect. Related references: PMID:18270207, PMID:19665976, PMID:22065636
• Upon GTP hydrolysis, the homodimeric complex undergoes conformational changes through its 3HB regions, pulling opposing membranes together to drive fusion; after phosphate release, the dimer disassembles to reset the cycle.
• Localized to the endoplasmic reticulum membrane via two transmembrane helices.
• Expressed in peripheral tissues at the protein level.
• Contains a coiled-coil domain and is subject to post-translational modifications including methylation and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 390–470 of human ATL2; consider using a blocking peptide from this region to confirm signal specificity.
• For Western blotting, expect a band near 66 kDa; verify in lysates from ATL2-expressing cell lines or tissues, and include appropriate positive/negative controls.
• In immunofluorescence/ICC, anticipate an ER-like reticular staining pattern; co-localization with an ER-specific marker can be used to validate localization.
• Evaluate antibody performance in your specific sample type and assay conditions prior to large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For studies on ER dynamics and membrane trafficking, CamelBio can supply this ATL2 antibody together with complementary reagents to support your workflow.