Anti-ATIC Rabbit Polyclonal Antibody for WB, ELISA - P31939

Core Product Specifications and Parameters

Biological Background: ATIC Function and Localization

• ATIC is a bifunctional enzyme that catalyzes the final two steps of de novo purine biosynthesis, converting AICAR to IMP via formyl-AICAR. Related references: PMID:11948179, PMID:14756554
• It possesses both AICAR transformylase and IMP cyclohydrolase activities, using 10-formyldihydrofolate or 10-formyltetrahydrofolate as formyl donors. Related references: PMID:10985775
• The enzyme can also convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of leukemia. Related references: PMID:10985775
• ATIC is predominantly localized in the cytoplasm and cytosol.
• It is widely expressed in tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• The gene undergoes alternative splicing, and post-translational modifications such as acetylation have been identified.
• ATIC promotes insulin receptor autophosphorylation and internalization, suggesting a role in insulin signaling. Related references: PMID:25687571

Experimental Guidance and Technical Tips

• The immunogen (aa 1-300) corresponds to the N‑terminal AICAR transformylase domain. Consider using this antibody for Western blotting of endogenous ATIC in human cell lines (predicted band ~65 kDa).
• For ELISA, validate performance with recombinant ATIC protein or cell lysates. Non‑specific binding may be reduced by optimizing blocking conditions.
• Species cross‑reactivity is reported as human; testing in other species may require sequence alignment and experimental verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We support purine metabolism and metabolic disease research by sourcing targets like ATIC, providing tailored solutions from concept to clinic. Our expert team ensures reliable supply and technical consultation for diagnostic development and beyond.