Anti-ATG9B Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q674R7

Core Product Specifications and Parameters

Biological Background: ATG9B Function and Localization

• ATG9B (also known as APG9-like 2 or protein sONE) is a multi-pass transmembrane protein encoded by the ATG9B gene on human chromosome 7.
• It functions as a phospholipid scramblase that plays a critical role in autophagy by mediating autophagosomal membrane expansion.
• The scramblase activity distributes phospholipids from the cytoplasmic to the luminal leaflet of the phagophore, a process that relies on lipid delivery by ATG2 proteins (ATG2A or ATG2B).
• Beyond autophagy, ATG9B is also implicated in necrotic cell death.
• Subcellularly, ATG9B localizes to the membrane of the preautophagosomal structure (phagophore assembly site).
• Tissue expression is predominantly high in placental trophoblast cells and pituitary gland, while absent in vascular endothelial cells.
• The protein belongs to the ATG9 family, and its structure includes multiple transmembrane helices; it is a key component of the autophagy machinery.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the C-terminal region (amino acids 715-924) of human ATG9B; the antibody may detect full-length ATG9B as well as any C-terminal fragments.
• For Western blotting, a predicted band around 101 kDa is expected; consider using a 6-8% SDS-PAGE gel for optimal resolution and ensure complete transfer of high-molecular-weight proteins.
• Paraffin-embedded immunofluorescence (IF-P) may require antigen retrieval; validate staining in tissues with known ATG9B expression (e.g., placenta) and include appropriate controls.
• As a polyclonal antibody, lot-to-lot variability is possible; it is recommended to titrate and validate each new lot individually in your specific assay system.
• Cross-reactivity with mouse and rat ATG9B is indicated; confirm sequence homology in the immunogen region for these species if critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting diagnostic developers and researchers with high-quality antibodies, validated antibody pairs, and bulk ancillary reagents. For autophagy and cell death studies, we offer this anti-ATG9B polyclonal antibody alongside custom monoclonal development services to accelerate your assay pipeline. Our supply chain expertise ensures consistent access to rare-target reagents and technical consultation from concept to clinic.