Anti-ATG9A Polyclonal Antibody for WB - Q7Z3C6

Core Product Specifications and Parameters

Biological Background: ATG9A Function and Localization

• ATG9A (Autophagy-related protein 9A), also known as mATG9 and APG9-like 1, is encoded by the ATG9A gene.
• Functions as a lipid scramblase essential for autophagy, mediating autophagosomal membrane expansion. Related references: PMID:22456507, PMID:27510922, PMID:29437695
• Cycles between the phagophore assembly site and cytoplasmic vesicles to supply membrane for autophagosome growth. Related references: PMID:16940348, PMID:22456507, PMID:33106659
• Its scramblase activity distributes ATG2-delivered phospholipids to drive membrane expansion. Related references: PMID:33106659
• Recruits phosphatidylinositol 4-kinase beta (PI4KB) via ARFIP2 to supply PI4P at the autophagosome initiation site. Related references: PMID:30917996
• Also implicated in necrotic cell death pathways (By similarity).
• Subcellularly localizes to preautophagosomal structures, autophagosome membranes, Golgi, late endosomes, recycling endosomes, ER, and mitochondria.
• Post-translational modifications include acetylation, phosphorylation, ubiquitination, and glycosylation.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~94 kDa; consider using appropriate gel percentage and blocking conditions. Validate in relevant sample systems.
• The immunogen covers a region (aa 180-290) conserved across human, mouse, and rat, supporting predicted cross-reactivity. Confirm specificity in your experimental model.
• Suitable for ELISA; consider optimizing coating antigen and detection conditions.
• As a polyclonal antibody, it may recognize multiple epitopes; evaluate lot-to-lot consistency for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-ATG9A polyclonal antibody supports autophagy research applications; for custom or rare-target raw-material sourcing, our team offers comprehensive procurement and supply-chain support to accelerate your development pipeline.