Anti-ATG8 Rabbit Polyclonal Antibody for WB, ELISA - Q7XY24

Core Product Specifications and Parameters

Biological Background: ATG8 Function and Localization

• ATG8 is an autophagy-related protein in wheat (Triticum aestivum), encoded by the gene LOC123044698.
• It belongs to the ATG8/LC3 family, a group of ubiquitin-like proteins that play a central role in autophagosome formation and maturation.
• The protein localizes to cytoplasmic vesicles, the autophagosome membrane, and the vacuole membrane, reflecting its dynamic involvement in the autophagy pathway.
• ATG8 is annotated as a lipoprotein and a component of the Ubl conjugation pathway, indicating that it undergoes C-terminal lipidation for membrane anchoring.
• Keywords from the UniProt entry (Q7XY24) include: Autophagy, Cytoplasmic vesicle, Lipoprotein, Membrane, Reference proteome, Ubl conjugation pathway, Vacuole.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1–100) of wheat ATG8; consider this region's conservation when assessing cross-reactivity with other plant species.
• For Western blot analysis, expect a band migrating at approximately 14 kDa, consistent with the protein's predicted molecular weight; validate the signal using appropriate positive and negative controls.
• The antibody has demonstrated cross-reactivity with Arabidopsis thaliana and Zea mays in ELISA and WB; further validation is recommended in your specific sample system.
• As a polyclonal antibody, it may recognize multiple ATG8 isoforms or processed forms; confirm isoform specificity if required for your assay.
• For ELISA applications, optimize coating antigen, antibody dilutions, and blocking conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing platform for IVD raw materials, connecting diagnostic developers with high-quality reagents like this anti-ATG8 polyclonal antibody to support plant autophagy research. Our offerings encompass validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibody development, ensuring seamless procurement from discovery through commercialization.