Anti-ATG7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95352

Core Product Specifications and Parameters

Biological Background: ATG7 Function and Localization

• ATG7 is an E1-like activating enzyme essential for two ubiquitin-like conjugation systems that drive autophagy and cytoplasm-to-vacuole transport (Cvt).
• It activates ATG12 for conjugation with ATG5, and primes ATG8 family proteins (such as LC3) for lipidation with phosphatidylethanolamine, a step critical for autophagosome membrane association.
• ATG7 facilitates the conversion of LC3-I to lipidated LC3-II on autophagosomal membranes, a key marker of autophagic flux. Related references: PMID:34161705
• Beyond canonical autophagy, ATG7 is required for mitophagy, helping to maintain mitochondrial homeostasis and prevent excess reactive oxygen species (ROS) production.
• It modulates p53/TP53 activity to influence cell cycle and survival under metabolic stress, and participates in processes such as axonal homeostasis, hematopoietic stem cell maintenance, Paneth cell granule formation, and adipose differentiation.
• ATG7 also contributes to circadian rhythm regulation by mediating the autophagic degradation of the clock repressor CRY1, linking autophagy to the liver clock and glucose metabolism.
• The protein localizes predominantly to the cytoplasm and preautophagosomal structures, where it orchestrates autophagy-related conjugation events.
• ATG7 is widely expressed, with highest levels observed in kidney, liver, lymph nodes, and bone marrow.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 500–676 of human ATG7, a region near the C-terminus that is well-conserved among mammals. This design may contribute to cross-reactivity with mouse and rat ATG7.
• For Western blot (WB), a band around 78 kDa is expected; consider loading adequate protein and optimizing transfer conditions to capture the full-length protein.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), ATG7 staining may appear diffuse in the cytoplasm with potential punctate enrichment at preautophagosomal structures under autophagy-inducing conditions. Validate with autophagy markers (e.g., LC3) in the relevant tissue or cell model.
• For ELISA applications, the antibody may serve as a capture or detection reagent; however, pairing with a suitable matched antibody and optimizing coating conditions are advised for best performance.
• Given ATG7’s central role in autophagy, this antibody can be useful for monitoring autophagy flux, mitophagy, and related pathways. Always include appropriate controls (e.g., autophagy inhibitors like chloroquine) to ensure specificity for autophagic degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in autophagy and related cellular pathways, we support the sourcing of ATG7 antibodies and other critical reagents for research and assay development. From concept to clinic, CamelBio streamlines supply chains to help you accelerate your projects.