Anti-ATG7 Polyclonal Antibody for IHC-P and ELISA - O95352

Core Product Specifications and Parameters

Biological Background: ATG7 Function and Localization

• E1-like activating enzyme essential for the two ubiquitin-like conjugation systems required for autophagy and cytoplasm-to-vacuole transport.
• Activates ATG12 for conjugation with ATG5, and the ATG8 family for conjugation with phosphatidylethanolamine, driving autophagosome formation.
• Facilitates LC3-I lipidation to generate LC3-II, which localizes to autophagosomal membranes. Related references: PMID:34161705
• Localizes to the cytoplasm and preautophagosomal structures, consistent with its role in early autophagy steps.
• Widely expressed across human tissues, notably enriched in kidney, liver, lymph nodes, and bone marrow.
• Regulates diverse processes including mitophagy, axonal homeostasis, hematopoietic stem cell maintenance, Paneth cell granule formation, and adipose differentiation.
• Post-translationally modified by acetylation and phosphorylation; belongs to the ubiquitin-activating E1-like protein family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–676 in the C-terminal region of human ATG7; consider epitope accessibility in your assay design.
• For IHC-P, optimize antigen retrieval and antibody dilution using human tissue sections with known ATG7 expression (e.g., kidney, liver).
• In ELISA, validate coating conditions with recombinant ATG7 protein or peptide and include appropriate positive/negative controls.
• Since cross-reactivity is limited to human, verify performance in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering one-stop access to validated IVD raw materials. Our ATG7 polyclonal antibody supports research in autophagy and related biomarker development, and we can supply bulk quantities, custom formulations, and complementary reagents to streamline your workflow from concept to clinical application.