Anti-ATG5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H1Y0

Core Product Specifications and Parameters

Biological Background: ATG5 Function and Localization

• ATG5 is a central autophagy protein that conjugates with ATG12 to form an E3-like enzyme complex, essential for ATG8 family lipidation and autophagosome formation.
• Besides autophagy, ATG5 participates in mitochondrial quality control, lymphocyte development and survival, MHC class II antigen presentation, axon maintenance, adipocyte differentiation, and primary ciliogenesis.
• ATG5 localizes to the cytoplasm and preautophagosomal structure membranes, consistent with its role in autophagic vesicle formation.
• It is ubiquitously expressed at the mRNA level, but the protein is dramatically upregulated in apoptotic cells, where it mediates caspase-dependent cell death.
• In apoptosis, ATG5 interacts with FADD and is required for IFN-gamma-induced autophagic cell death.
• In viral infection, ATG5 may act as a proviral factor: it is required for translation of incoming hepatitis C virus (HCV) RNA and negatively regulates innate antiviral immune responses via the type I IFN pathway. Related references: PMID:17709747 PMID:19666601
• ATG5 undergoes post-translational modifications including acetylation and the formation of an isopeptide bond during conjugation with ATG12, which is critical for its autophagic function.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 28-275 of human ATG5, a region involved in ATG12 conjugation; confirm that the antibody detects both unconjugated and conjugated forms if needed.
• For Western blot, expect a band at approximately 32 kDa in lysates from human, mouse, or rat tissues; include appropriate positive and negative controls.
• For IHC-P, optimize antigen retrieval and antibody dilution for each tissue type, as ATG5 expression levels can vary between tissues and apoptotic conditions.
• ELISA applications may require careful coating conditions; consider using recombinant ATG5 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supply partner, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our anti-ATG5 polyclonal antibody supports autophagy and cell death research, and we can source rare-target reagents tailored to your diagnostic development needs. From concept to clinic, CamelBio streamlines your procurement process.