Anti-ATG4D Polyclonal Antibody for WB and ELISA - Q86TL0

Core Product Specifications and Parameters

Biological Background: ATG4D Function and Localization

• ATG4D is a cysteine protease (autophagin-4) belonging to the ATG4 family, which includes ATG4A, ATG4B, and ATG4C; it primarily mediates delipidation of ATG8 family proteins.
• Functions in autophagy by both proteolytic activation and delipidation of ATG8 proteins (MAP1LC3 and GABARAPL2); cleaves the C-terminal amino acid to expose a glycine necessary for conjugation to phosphatidylethanolamine (PE) and membrane insertion. Related references: PMID:21177865
• Mediates delipidation of PE-conjugated ATG8 proteins during macroautophagy and also delipidates ATG8 proteins conjugated to phosphatidylserine (PS) during non-canonical autophagy at endolysosomal compartments. Related references: PMID:29458288 PMID:33909989
• Plays a role in mitophagy by regulating ATG9A trafficking to mitochondria and promoting phagophore-ER contacts independently of its protease activity. Related references: PMID:33773106
• Links mitochondrial dysfunction to apoptosis: mitochondrial import of ATG4D during stress regulates mitochondrial physiology, ROS, mitophagy, and cell viability.
• Localizes to the cytoplasm and mitochondrial matrix.
• Post-translationally regulated by phosphorylation; contains alternative splicing variants.
• Widely expressed, with highest expression in testis.

Experimental Guidance and Technical Tips

• The immunogen (aa 265-474) corresponds to a region that may confer specificity for ATG4D over other ATG4 family members; verify specificity using positive and negative controls.
• For Western blot, validate the antibody in human and rat cell or tissue lysates; a band around 53 kDa is expected. Consider using reducing conditions.
• ELISA applications may be optimized by using this polyclonal antibody as a detection antibody in a sandwich ELISA format with a suitable capture antibody.
• Since ATG4D is involved in autophagy and apoptosis, experimental conditions (e.g., starvation, mitochondrial stress) may affect expression levels; include appropriate treatments.

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CamelBio provides diagnostic manufacturers and research institutes with reliable sourcing for raw materials like this ATG4D polyclonal antibody, supporting autophagy and apoptosis research. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents tailored to your assay development needs. For rare or custom targets, our sourcing network ensures timely access to high-quality materials from concept to clinic.