Anti-ATG4A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WYN0

Core Product Specifications and Parameters

Biological Background: ATG4A Function and Localization

• ATG4A is a cysteine protease essential for autophagy, mediating both proteolytic activation and delipidation of ATG8 family proteins. Related references: PMID:12473658, PMID:15169837, PMID:17347651

• The protease activity of ATG4A cleaves the C-terminal residue of ATG8 proteins (including MAP1LC3A, GABARAP, GABARAPL2) to expose a glycine, which is required for their conjugation to PE and membrane targeting during autophagy. Related references: PMID:12473658, PMID:15169837, PMID:17347651

• ATG4A catalyzes delipidation of PE-conjugated ATG8 family proteins, with stronger delipidation activity relative to ATG4B, thereby contributing to recycling of ATG8 proteins. Related references: PMID:29458288, PMID:33909989

• It functions as a deubiquitinating-like enzyme to counteract ATG8ylation, cleaving ATG8 from conjugates like ATG3. Related references: PMID:31315929, PMID:33773106

• Independently of its protease activity, ATG4A regulates mitophagy by controlling ATG9A trafficking to mitochondria and facilitating phagophore-endoplasmic reticulum contacts during lipid transfer. Related references: PMID:33773106

• ATG4A localizes to the cytoplasm, consistent with its role in autophagy.

• The ATG4A gene undergoes alternative splicing, producing multiple isoforms, and is identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 210–398 of human ATG4A, a region that may include functionally relevant domains; epitope preservation and species homology should be considered when interpreting cross-reactivity data.
• For Western blotting under reducing conditions, a band near 45 kDa is anticipated; use appropriate cellular or tissue lysates with confirmed ATG4A expression as positive controls.
• In immunofluorescence or immunocytochemistry, ATG4A cytoplasmic localization should be detectable; optimize fixation and permeabilization protocols for the specific cell type.
• ELISA applications can be developed using recombinant ATG4A protein or peptide fragments; assay conditions may require empirical optimization.
• Cross-reactivity with mouse and rat orthologs is stated; validate specificity in the target species using knockout or knockdown models where feasible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive IVD raw-material solutions, from discovery through clinical application, supporting every phase of diagnostic development. For autophagy targets such as cysteine protease ATG4A, we offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with CamelBio to streamline your assay development with reliable supply and technical expertise.