Anti-ATG16L2 Polyclonal Antibody for WB and ELISA - Q8NAA4

Core Product Specifications and Parameters

Biological Background: ATG16L2 Function and Localization

• ATG16L2 (Autophagy-related protein 16-2), also known as APG16-like 2 or WD repeat-containing protein 80 (WDR80), is encoded by the human ATG16L2 gene.
• It is a member of the ATG16 family and contains WD repeats, which are involved in protein-protein interactions.
• The protein is predicted to feature coiled-coil domains typical of autophagy-related factors.
• ATG16L2 may contribute to the regulation of epithelial homeostasis, functioning in an ATG16L1-dependent manner.
• Subcellular fractionation and proteomics indicate localization in the cytoplasm and cytosol.
• Keywords associated with ATG16L2 include protein transport, alternative splicing, and cytoplasmic localization.
• It has been identified in reference proteome and proteomics identification studies.

Experimental Guidance and Technical Tips

• Given the immunogen is a recombinant fragment covering the N-terminal region (aa 1-275), this antibody may recognize both full-length ATG16L2 and potentially truncated isoforms. Consider performing epitope mapping if isoform-specific detection is required.
• For WB, the predicted molecular weight is 69 kDa; ensure appropriate gel percentage and transfer conditions for this size. Use a positive control lysate from human, mouse, or rat tissues known to express ATG16L2.
• For ELISA, titrate the antibody to determine optimal coating and detection concentrations using the immunogen or a relevant peptide.
• Since cross-reactivity is reported for human, mouse, and rat, validate specificity in the target species by knockdown or knockout controls if available.
• This antibody is suitable for studying autophagy-related processes and epithelial homeostasis; consider co-staining with autophagy markers like LC3 or ATG16L1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with reliable raw materials for autophagy and cell biology investigations. From validated antibody pairs to bulk ancillary reagents, we streamline procurement of high-quality tools for studying ATG16L2 and related targets. Contact our team for customized sourcing solutions tailored to your assay development needs.