Anti-ATG16L1 Rabbit Monoclonal Antibody for WB, ELISA - Q676U5

Core Product Specifications and Parameters

Biological Background: ATG16L1 Function and Localization

• ATG16L1, also known as APG16-like 1, is a core autophagy protein characterized by WD repeat and coiled-coil domains.
• It interacts with the ATG12-ATG5 conjugate to mediate lipidation of ATG8 family proteins (MAP1LC3A/B/C, GABARAPL1/2, GABARAP), a critical step for autophagosomal membrane elongation. Related references: PMID:23376921 PMID:23392225 PMID:24553140
• In addition to canonical autophagy, ATG16L1 is involved in non-canonical autophagy by mediating conjugation of ATG8 proteins to single membranes at endolysosomal compartments, possibly via phosphatidylserine. Related references: PMID:33909989
• Acts as a molecular hub coordinating distinct autophagy pathways through different domains, enabling both canonical and non-canonical signaling. Related references: PMID:29317426 PMID:30778222
• Negatively regulates NOD1- and NOD2-driven inflammatory cytokine responses while promoting autophagy-dependent antibacterial pathways together with NOD1 or NOD2. Related references: PMID:24238340 PMID:20637199
• Modulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production, linking autophagy to innate immunity. Related references: PMID:22749352 PMID:25645662
• Plays a role in maintaining Paneth cell morphology and function, highlighting its importance in intestinal homeostasis. Related references: PMID:18849966
• Subcellularly localizes to the cytoplasm and associates with membranes of preautophagosomal structures, endosomes, and lysosomes.
• Contains WD repeat and coiled-coil domains and is subject to phosphorylation, as indicated by its keywords.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is approximately 68 kDa. Post-translational modifications or protein complexes may cause additional bands; validate using appropriate controls.
• Validate antibody specificity with ATG16L1 knockout (KO) cell lines or siRNA knockdown, as the antibody is KO-validated.
• For ELISA, consider using the antibody as a capture or detection reagent, and optimize coating concentrations and buffer conditions for your assay.
• Since the immunogen spans amino acids 6-105 near the N-terminus, this antibody may detect multiple isoforms or cleavage products; confirm reactivity in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For autophagy research, we offer anti-ATG16L1 antibodies, optimized for applications like WB and ELISA, along with bulk sourcing options and technical support. Contact us to discuss your specific requirements.