Anti-ATG16L1 Polyclonal Antibody - Q676U5

Core Product Specifications and Parameters

Biological Background: ATG16L1 Function and Localization

• ATG16L1 is a central autophagy protein that interacts with the ATG12-ATG5 conjugate to mediate lipidation of ATG8 family proteins (LC3s and GABARAPs), a critical step for autophagosome membrane elongation.
  Related references: PMID:23376921, PMID:23392225, PMID:24553140

• It acts as a molecular hub, coordinating both canonical autophagy (conjugation of phosphatidylethanolamine to ATG8 on double membranes) and non-canonical autophagy (conjugation of phosphatidylserine to ATG8 on single membranes, such as endolysosomes) through distinct structural domains.
  Related references: PMID:29317426, PMID:30778222, PMID:33909989

• ATG16L1 negatively regulates NOD1- and NOD2-driven inflammatory cytokine production while simultaneously promoting NOD-dependent antibacterial autophagy.
  Related references: PMID:24238340, PMID:20637199

• It modulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon production, connecting autophagy to innate antiviral immunity.
  Related references: PMID:22749352, PMID:25645662

• ATG16L1 is essential for normal Paneth cell morphology and function in the intestinal epithelium, with dysregulation linked to inflammatory bowel disease.
  Related references: PMID:18849966

• The protein localizes to the cytoplasm, preautophagosomal structures, endosomes, and lysosomes, and contains multiple WD40 repeats and a coiled-coil domain.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 90-300) spans the ATG5-interaction domain, conserved in human, mouse, and rat. Given the polyclonal nature, multiple epitopes may enhance detection sensitivity.
• For Western blotting, a band of approximately 68 kDa is anticipated. Use reducing conditions and validate specificity in your experimental system.
• In IHC-P, perform antigen retrieval optimization; strong staining may be observed in Paneth cells and other autophagy-active tissues.
• For IF/ICC, consider ATG16L1’s punctate cytoplasmic and membrane association; fixation and permeabilization conditions should be empirically determined.
• ELISA applications may require pairing validation; the antibody can be explored as both capture and detection reagent.
• As with all polyclonal antibodies, lot-to-lot consistency should be monitored, and new lots validated in relevant assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material solutions, from validated polyclonal and monoclonal antibodies to bulk ancillary reagents. This anti-ATG16L1 antibody is part of our expanding autophagy research portfolio, supporting studies in inflammatory bowel disease, host-pathogen interactions, and innate immunity. With a commitment to streamlined procurement, we assist diagnostic manufacturers and labs in sourcing rare-target reagents, antibody pairs, and customized assay components to accelerate your development workflow.