Anti-ATG16L1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q676U5

Core Product Specifications and Parameters

Biological Background: ATG16L1 Function and Localization

• ATG16L1 is a central autophagy hub that coordinates both canonical and non-canonical autophagy pathways through distinct functional domains. Related references: PMID:29317426 PMID:30778222
• In canonical autophagy, it forms a complex with ATG12-ATG5 to catalyze phosphatidylethanolamine (PE) conjugation to ATG8 family proteins (LC3/GABARAP), driving autophagosomal membrane elongation. Related references: PMID:23376921 PMID:23392225 PMID:24553140
• ATG16L1 also participates in non-canonical autophagy, likely catalyzing phosphatidylserine (PS) conjugation to ATG8 on single membranes, a process critical for limiting influenza A virus infection in epithelial cells. Related references: PMID:33909989
• The protein localizes to the cytoplasm, and associates with preautophagosomal structures, endosomes, and lysosomes, consistent with its roles in membrane trafficking.
• ATG16L1 modulates innate immune signaling: it regulates MAVS-dependent type I interferon production and negatively regulates NOD1/NOD2-driven inflammatory cytokine responses. Related references: PMID:22749352 PMID:25645662 PMID:24238340
• Together with NOD1 and NOD2, it promotes an autophagy-dependent antibacterial pathway, and is essential for maintaining Paneth cell morphology and function in the intestinal epithelium. Related references: PMID:20637199 PMID:18849966
• ATG16L1 contains WD repeats and a coiled-coil domain, undergoes phosphorylation and alternative splicing, and is identified in various proteomic screens.

Experimental Guidance and Technical Tips

• Given the immunogen corresponds to amino acids 200–300 of human ATG16L1 (NP_110430.5), the antibody is predicted to detect full-length ATG16L1 and cross-react with the specified species (human, mouse, rat). Confirm specificity in the target sample system.
• For Western blot (WB), the expected band is ~68 kDa; use appropriate positive controls and validate band identity by molecular weight markers or knockdown/knockout models.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and antigen retrieval to preserve the epitope; ATG16L1’s cytoplasmic and punctate membrane localization may require careful permeabilization.
• When using ELISA, the antibody can serve as either capture or detection reagent; empirical optimization of coating concentration and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined, one-stop access to high-quality IVD raw materials, technical services, and consultative support—from concept to clinic. This ATG16L1 polyclonal antibody supports autophagy and innate immunity research and assay development. Complement your workflow with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets.