Anti-ATG14 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6ZNE5

Core Product Specifications and Parameters

Biological Background: ATG14 Function and Localization

• ATG14 (also known as Atg14L or Barkor) is a key autophagy regulator required for both basal and inducible autophagy. It functions as part of the autophagy-specific PI3-kinase complex PI3KC3-C1, determining its localization. Related references: PMID:18843052, PMID:19050071
• ATG14 promotes autophagosome formation and the conjugation of MAP1LC3/LC3 to phosphatidylethanolamine, a critical step in autophagosome elongation. Related references: PMID:19270696, PMID:20713597
• ATG14 promotes the translocation of BECN1 from the trans-Golgi network to autophagosomes. Related references: PMID:20713597
• ATG14 enhances PIK3C3 activity in a BECN1-dependent manner.
• ATG14 is essential for the autophagy-dependent phosphorylation of BECN1 and stimulates BECN1 phosphorylation while suppressing AMPK-mediated phosphorylation of PIK3C3. Related references: PMID:23878393
• It binds to the STX17-SNAP29 t-SNARE complex on autophagosomes and primes it for VAMP8 interaction, promoting autophagosome-endolysosome fusion. Related references: PMID:25686604, PMID:37632749
• ATG14 localizes to the cytoplasm, endoplasmic reticulum membrane, preautophagosomal structure membrane, and autophagosome membrane, reflecting its roles in autophagosome biogenesis and maturation.
• ATG14 is a coiled-coil protein that undergoes alternative splicing; it is phosphorylated and ubiquitinated, as indicated by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 340–440) of human ATG14, which is conserved across species, supporting cross-reactivity with mouse and rat. Validate in the relevant sample system.
• For Western blotting, a band at approximately 55 kDa is expected; consider using whole-cell lysates or subcellular fractions for autophagy induction experiments.
• IF/ICC protocols should include permeabilization to detect membrane-localized ATG14 on autophagosomes and other compartments; appropriate negative and positive controls are recommended.
• ELISA applications may require optimization of coating antigen concentration and antibody dilution; include a standard curve for quantitative assessment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials—from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents. For autophagy studies, this anti-ATG14 polyclonal antibody is part of our portfolio, and we stand ready to fulfill custom or large-scale sourcing needs. From concept to clinic, CamelBio streamlines the procurement of critical raw materials along with the technical support that accelerates your development pipeline.