Anti-ATG13 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75143

Core Product Specifications and Parameters

Biological Background: ATG13 Function and Localization

• ATG13 (Autophagy-related protein 13), also known as KIAA0652 or PARATARG8, is a key autophagy factor required for autophagosome formation and mitophagy.
• It functions as a target of the TOR kinase signaling pathway, which regulates autophagy through phosphorylation of ATG13 and ULK1, and formation of the ATG13-ULK1-RB1CC1 complex.
• ATG13, together with ULK1, modulates the kinase activity of mTORC1 and contributes to the regulation of cell proliferation.
• Subcellularly, ATG13 localizes to the cytoplasm, cytosol, and preautophagosomal structures, consistent with its role in early autophagosome nucleation.
• The protein is subject to post-translational modifications including phosphorylation (critical for its autophagy-regulatory function) and acetylation.
• Multiple isoforms are produced by alternative splicing, as indicated by UniProt keywords, though specific tissue-specific or functional differences remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 281-480) of human ATG13, which may recognize multiple isoforms and species orthologs given the sequence homology.
• For Western blotting, the expected molecular weight is approximately 57 kDa; however, due to alternative splicing and post-translational modifications, additional bands may be observed.
• In immunofluorescence/immunocytochemistry, ATG13 typically exhibits a diffuse cytoplasmic/punctate pattern; under autophagy induction (e.g., starvation or rapamycin treatment), punctate signals may increase, indicating preautophagosomal structure localization.
• ELISA applications can be developed using this antibody as a capture or detection reagent; optimization of coating conditions and detection system is recommended.
• As a polyclonal antibody, it is advisable to validate specificity in the relevant sample system, and consider using blocking with immunogen or peptide competition if non-specific binding is suspected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material sourcing, including this high-quality ATG13 rabbit polyclonal antibody for autophagy and mitophagy research. We support diagnostic manufacturers and laboratories with one-stop access to validated antibodies, custom development services, and bulk ancillary reagents, accelerating assay development from concept to clinic.