Anti-ATG12 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - O94817

Core Product Specifications and Parameters

Biological Background: ATG12 Function and Localization

• ATG12 (Autophagy-related protein 12) is a ubiquitin-like protein belonging to the autophagy-related (ATG) family, essential for autophagosome formation.
• It is conjugated to ATG5 via a ubiquitin-like conjugation system involving ATG7 (E1-like) and ATG10 (E2-like), forming an ATG12-ATG5 conjugate that acts as an E3-like enzyme for ATG8 family lipidation, a critical step in autophagosome membrane expansion.
• The ATG12-ATG5 conjugate, in complex with ATG16L1, is required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity under lysosomal stress conditions.
• ATG12 is localized to the cytoplasm and preautophagosomal structure membrane.
• Post-translational modifications include acetylation and formation of an isopeptide bond during conjugation; alternative splicing generates multiple isoforms.
• ATG12 plays a role in host-virus interactions: it acts as a proviral factor for hepatitis C virus (HCV) by supporting translation of incoming viral RNA, and it negatively regulates the innate antiviral immune response against vesicular stomatitis virus (VSV) by impairing type I interferon production. Related references: PMID:17709747, PMID:19666601
• Tissue expression is ubiquitous.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human ATG12, which may recognize both unconjugated ATG12 (15 kDa) and the ATG12-ATG5 conjugate (~55 kDa). For Western blot, consider using reducing conditions to distinguish between these forms.
• For IHC-P, antigen retrieval may be required; validate in tissue known to express ATG12 (ubiquitous expression, but autophagy upregulation conditions may enhance staining).
• For IP, the antibody is suitable for capturing native ATG12 complexes; ensure proper lysis conditions to preserve protein interactions.
• ELISA applications can be developed using this antibody as capture or detection reagent; test cross-reactivity with mouse ATG12 if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions for autophagy research, including validated ATG12 antibodies, recombinant proteins, and ancillary reagents. Our one-stop sourcing platform supports diagnostic assay development from concept to clinic, ensuring reliable supply of critical components like monoclonal antibodies and bulk reagents.