Anti-ATG12 Rabbit Monoclonal Antibody for WB, ELISA - O94817

Core Product Specifications and Parameters

Biological Background: ATG12 Function and Localization

• ATG12 is a ubiquitin-like protein essential for autophagy vesicle formation. It conjugates with ATG5 through a ubiquitin-like system involving ATG7 (E1-like) and ATG10 (E2-like), and the ATG12-ATG5 conjugate acts as an E3-like enzyme for ATG8 lipidation and membrane association (By similarity).
• The ATG12-ATG5-ATG16L1 complex recruits LRRK2 to stressed lysosomes, inducing LRRK2 kinase activity upon lysosomal stress (By similarity).
• ATG12 may act as a proviral factor: in association with ATG5, it negatively regulates innate antiviral immunity by impairing type I IFN production upon VSV infection. Related references: PMID:17709747
• It is required for translation of incoming hepatitis C virus (HCV) RNA to initiate replication, but not for maintenance once infection is established. Related references: PMID:19666601
• ATG12 localizes to the cytoplasm and preautophagosomal structure membranes, consistent with its role in autophagosome formation.
• The protein is ubiquitously expressed across human tissues.
• Post-translational modifications include acetylation and isopeptide bond formation as part of the Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 41-140 of human ATG12, which may recognize both free ATG12 and the ATG12-ATG5 conjugate. Consider this when interpreting results in autophagy-related assays.
• For Western blot, validate detection around 15 kDa (or 55-60 kDa for the conjugate) in human and mouse cell lysates. Use appropriate controls for autophagy induction (e.g., starvation or lysosomal stress).
• For ELISA, coating with the immunogen peptide or recombinant ATG12 protein may improve assay performance. Assess cross-reactivity with ATG5 and other ubiquitin-like proteins if specificity is critical.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutions with validated antibodies, bulk reagents, and rare target procurement. This ATG12 monoclonal antibody is suitable for autophagy-related research and assay development, and CamelBio can assist with complementary antibody pairs, ancillary reagents, and scalable supply solutions tailored to your diagnostic needs.