Anti-ATG10 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H0Y0

Core Product Specifications and Parameters

Biological Background: ATG10 Function and Localization

• ATG10 is a ubiquitin-like-conjugating enzyme (E2-like) essential for autophagy.
• It catalyzes the conjugation of ATG12 to ATG5, a critical step for autophagosome formation.
• ATG10 likely serves as an ATG5-recognition molecule and is not involved in ATG12 conjugation to ATG3.
• The protein plays a role in adenovirus-mediated cell lysis.
• Subcellular localization: predominantly cytoplasmic.
• Alternative splicing produces multiple isoforms.
• Keywords: Autophagy, Cytoplasm, Protein transport, Transferase, Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 120–220 of human ATG10, potentially detecting both denatured and native protein; optimize conditions for each application.
• For Western blotting, the predicted molecular weight is 25 kDa. Validate band specificity using positive and negative controls, especially across species.
• In IHC-P, antigen retrieval may be required; test citrate or EDTA-based heat-induced epitope retrieval for formalin-fixed tissues.
• Expected cross-reactivity with mouse and rat should be confirmed in your specific samples before large-scale experiments.
• For ELISA, the antibody can be used as a capture or detection reagent; consider pairing with ATG5 or ATG12 antibodies for pathway studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, supporting autophagy research with validated antibodies. For ATG10 and related targets, we supply optimized polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate your assay development from concept to clinic.