Anti-ATG10 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9H0Y0

Core Product Specifications and Parameters

Biological Background: ATG10 Function and Localization

• ATG10 is an E2-like enzyme essential for autophagy, functioning as the conjugating enzyme for ATG12 attachment to ATG5.
• The ATG12–ATG5 conjugate is a crucial component of the autophagy machinery, required for autophagosome formation.
• ATG10 likely acts as an ATG5-recognition molecule, ensuring specific conjugation of ATG12 to ATG5 rather than other substrates.
• It is not involved in ATG12 conjugation to ATG3, highlighting substrate specificity within the ubiquitin-like conjugation system.
• ATG10 plays a role in adenovirus-mediated cell lysis, suggesting relevance in host-pathogen interactions.
• Subcellular localization is predominantly cytoplasmic, consistent with its role in early autophagic processes.
• The protein undergoes alternative splicing, yielding isoforms that may have distinct functional properties.
• Keywords associated with ATG10 include autophagy, protein transport, ubl conjugation pathway, and transferase activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 121–220 of human ATG10; confirm that the epitope is present in your target isoform and species of interest.
• This antibody is validated for WB, IF/ICC, and ELISA. For WB, a band around 25 kDa is expected. Optimize loading controls and antibody dilution for your specific lysates.
• Cross-reactivity with mouse ATG10 is reported, indicating sequence conservation in the immunogen region. Validate performance in mouse models if used.
• For IF/ICC, consider appropriate fixation and permeabilization methods to detect cytoplasmic ATG10. Include proper controls to assess specificity.
• Always titrate the antibody in your own assay system, as performance can vary with experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research institutes from concept to clinic. For autophagy researchers, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, ensuring access to critical targets like ATG10. Our rare-target sourcing capabilities help bridge gaps in your assay development pipeline, backed by technical consulting and reliable supply.