Anti-ATF7IP Rabbit Polyclonal Antibody for WB, ELISA - Q6VMQ6

Core Product Specifications and Parameters

Biological Background: ATF7IP Function and Localization

• The ATF7IP gene encodes Activating transcription factor 7-interacting protein 1 (ATF7IP), also known as ATF-interacting protein (ATF-IP), MBD1-containing chromatin-associated factor 1 (MCAF1), p621, and hAM. It functions as a transcriptional modulator that can act as both an activator and a repressor depending on the cellular context.
• ATF7IP is a recruiter that couples transcriptional factors to the general transcription apparatus, thereby modulating transcription regulation and chromatin formation. It is essential for HUSH-mediated heterochromatin formation and gene silencing. Related references: PMID:27732843
• It mediates MBD1-dependent transcriptional repression, likely by recruiting complexes that contain SETDB1. This complex represses transcription and couples DNA methylation to histone H3 'Lys-9' trimethylation (H3K9me3). Related references: PMID:12665582 PMID:14536086
• ATF7IP stabilizes SETDB1 and stimulates its histone methyltransferase activity, facilitating the conversion of dimethylated to trimethylated H3K9. This action reinforces heterochromatin formation and gene silencing.
• The protein plays a role in telomere maintenance by facilitating SP1-dependent expression of telomerase TERT and TERC genes in cancer cells, highlighting its involvement in oncogenesis. Related references: PMID:19106100
• ATF7IP is predominantly localized in the nucleus, consistent with its role in transcriptional regulation and chromatin modification.
• Tissue-specific expression analysis reveals low levels in normal breast, lung, and stomach tissues, but significant upregulation in the corresponding cancerous tissues, suggesting a potential role in tumorigenesis and indicating its utility as a cancer biomarker or therapeutic target.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation. Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 525-725 of human ATF7IP, a region that is likely to be recognized by this polyclonal antibody in denaturing Western blot conditions. Consider optimizing sample preparation and loading controls (e.g., nuclear extracts) for robust detection of the full-length 136 kDa protein.
• For Western blot, validate antibody specificity using positive and negative control lysates (e.g., wild-type vs. ATF7IP knockout cell lines, or normal vs. tumor tissues). Titrate the antibody to determine the optimal dilution for your experimental system.
• In ELISA applications, consider using recombinant ATF7IP protein or peptide as coating antigen to establish assay sensitivity. The antibody’s cross-reactivity with mouse ATF7IP allows flexibility in model organism studies, but sequence homology should be verified for the target region.
• Because ATF7IP is involved in multiple protein complexes (MBD1, SETDB1, SP1), co-immunoprecipitation or pull-down assays in combination with this antibody may be explored, but optimal conditions must be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-ATF7IP antibody supports studies in transcriptional regulation, chromatin biology, and cancer research. Beyond this polyclonal antibody, CamelBio can help you source validated antibody pairs, optimized monoclonal or recombinant antibodies, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development and production workflows.