Anti-ATF7 Rabbit Polyclonal Antibody for WB, ELISA - P17544

Core Product Specifications and Parameters

Biological Background: ATF7 Function and Localization

• ATF7 (Activating transcription factor 7) is a cyclic AMP-dependent transcription factor and stress-responsive chromatin regulator. It plays roles in innate immunological memory, adipocyte differentiation, and telomerase regulation. Related references: PMID:29490055
• In the absence of stress, ATF7 contributes to heterochromatin formation by recruiting histone H3K9 methyltransferases, silencing genes like STAT1 in adipocytes and innate immunity genes. Upon stress, phosphorylation disrupts this interaction, leading to transcriptional activation.
• ATF7 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter and negatively regulates ATF2 and ATF7 transcriptional activities by sequestering the Thr-53 phosphorylating kinase in the cytoplasm.
• Encoded by the ATF7 gene (GeneID: 11016), this protein contains a basic leucine zipper (bZIP) domain and a zinc finger motif, classifying it as a DNA-binding transcription factor.
• Subcellular localization includes nucleus, nucleoplasm, chromosome telomeres, and cytoplasm, reflecting its dynamic roles in both transcriptional regulation and telomere maintenance.
• ATF7 is expressed in various tissues, with highest levels in skeletal muscle, and lower expression in heart, brain, placenta, and lung, suggesting tissue-specific regulatory functions.
• Key post-translational modifications include phosphorylation, which modulates its activity, and ubiquitination (Ubl conjugation), as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against the N-terminal region (amino acids 1-117) of human ATF7. This region encompasses part of the transactivation domain and may recognize full-length ATF7 and potentially some splice variants or processed forms.
• For Western blot, the predicted molecular weight is approximately 52 kDa. It is advisable to use fresh lysates with protease and phosphatase inhibitors, as ATF7 is a phosphoprotein, and include appropriate loading controls.
• In ELISA, the polyclonal nature may provide robust signal; however, cross-reactivity with other ATF family members (e.g., ATF2) should be excluded using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to validated IVD antibodies like this anti-ATF7 polyclonal antibody, supporting studies in transcription regulation and stress response. Our portfolio includes optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your supply chain from discovery to commercial assay development.