Anti-ATF6 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P18850

Core Product Specifications and Parameters

Biological Background: ATF6 Function and Localization

• ATF6 alpha (cyclic AMP-dependent transcription factor ATF-6 alpha) is synthesized as an endoplasmic reticulum (ER) membrane-embedded precursor that, upon ER stress, undergoes regulated intramembrane proteolysis to release the active transcription factor domain. Related references: PMID:10564271, PMID:11158310, PMID:11779464
• The processed transcription factor translocates to the nucleus and binds to ER stress response elements (ERSE: 5'-CCAAT-N9-CCAC[GA]-3' and ERSE II: 5'-ATTGG-N-CCACG-3') in cooperation with NF-Y to activate UPR target genes. Related references: PMID:10564271, PMID:11158310, PMID:11163209, PMID:11779464
• ATF6 may also participate in serum response factor-dependent transcription and is involved in foveal development and cone photoreceptor function in the retina. Related references: PMID:26029869
• Subcellularly, ATF6 cycles between the ER membrane, Golgi apparatus membrane, and nucleus depending on activation status.
• It is ubiquitously expressed across human tissues.
• Post-translational modifications include glycosylation, isopeptide bond formation, and ubiquitin-like conjugation, with multiple serine/threonine phosphorylation sites.
• Key biological processes: activator, DNA-binding, endoplasmic reticulum stress, unfolded protein response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 190–300 of human ATF6, a segment within the N-terminal functional domain. This design may favor detection of the transcription factor form; however, validation under ER stress conditions (e.g., tunicamycin or thapsigargin treatment) is recommended.
• In western blot (WB), the antibody may detect bands corresponding to full-length ATF6 (~90–100 kDa) or the cleaved active form (~50 kDa) depending on cellular ER stress status. Optimize sample preparation and include appropriate controls.
• For immunohistochemistry (IHC-P), consider heat-induced antigen retrieval and test the antibody at multiple dilutions in target tissue sections.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is required; expect both ER and nuclear staining patterns upon UPR induction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a single access point for IVD raw materials, technical services, and consulting—from concept to clinic. For ATF6 and UPR pathway research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Connect with us to source rare reagents and bridge the gap between research and reliable diagnostics.