Anti-ATF6 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ChIP, ELISA - P18850

Core Product Specifications and Parameters

Biological Background: ATF6 Function and Localization

• ATF6, also known as activating transcription factor 6 alpha (ATF6-alpha), is a 75 kDa endoplasmic reticulum (ER) membrane-anchored precursor protein. It belongs to the ATF/CREB family of transcription factors. Related references: PMID:10564271, PMID:11158310, PMID:11779464
• Upon ER stress, ATF6 is translocated to the Golgi where it is cleaved, releasing its N-terminal cytoplasmic domain. The processed ATF6 fragment enters the nucleus and acts as a transcription factor to activate unfolded protein response (UPR) target genes. Related references: PMID:10564271, PMID:11158310, PMID:11779464
• ATF6 binds to ER stress response elements (ERSE) in the promoters of UPR genes, often requiring cooperation with NF-Y. It recognizes the consensus sequence 5'-CCAC[GA]-3' within ERSE motifs. Related references: PMID:10564271, PMID:11158310, PMID:11779464
• Subcellular localization is dynamic: ATF6 is found in the ER membrane and Golgi apparatus membrane under basal conditions; its active form translocates to the nucleus upon stress induction. Related references: PMID:10564271, PMID:11158310, PMID:11779464
• ATF6 is ubiquitously expressed and has been implicated in retinal development and cone function, with disease variants linked to achromatopsia. Related references: PMID:26029869
• Post-translational modifications include glycosylation and ubiquitination. The protein contains a transmembrane helix and is subject to regulated intramembrane proteolysis.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen used to generate this monoclonal antibody provides high target specificity; validate in your relevant model system.
• For Western blotting, a band of approximately 75 kDa is expected for full-length ATF6; consider using protease inhibitors to prevent degradation.
• In ChIP assays, optimize crosslinking and sonication conditions for nuclear ATF6 binding; validate with known UPR target genes.
• IHC-P may require antigen retrieval; test both enzymatic and heat-induced methods to ensure nuclear and/or ER staining detection.
• Cross-reactivity with mouse allows validation in rodent models of ER stress; always include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, bridging diagnostic developers with high-quality research reagents like this ATF6 antibody. We offer validated antibody pairs, bulk ancillary reagents, and tailored support for rare targets involved in cellular stress pathways. Whether you need monoclonal antibodies for UPR biomarker assays or custom sourcing for ER stress-related projects, CamelBio streamlines your supply chain from concept to clinic.