Anti-ATF5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, and ELISA - Q9Y2D1

Core Product Specifications and Parameters

Biological Background: ATF5 Function and Localization

• ATF5 (Activating transcription factor 5, also known as ATFX) is a transcription factor that binds to cAMP response element (CRE), ATF5-specific response element (ARE), and amino acid response element (AARE) to regulate gene expression (PubMed:10373550, PubMed:15358120, PubMed:20654631). Related references: PMID:10373550 PMID:15358120 PMID:20654631
• It is critically involved in cell survival, proliferation, and differentiation in a cell type-dependent manner. In the nervous system, ATF5 maintains neuroprogenitor cell proliferation and blocks neuronal differentiation; it must be down-regulated for differentiation to proceed.
• ATF5 activity is enhanced by CCND3 and inhibited by CDK4 (PubMed:15358120). Acetylation by EP300 promotes binding to ARE sequences on target genes like BCL2 and EGR1, contributing to anti-apoptotic functions (PubMed:21791614). Related references: PMID:15358120 PMID:21791614
• In the liver, ATF5 represses CRE-dependent transcription, inhibits proliferation via G2/M arrest (PubMed:18701499, PubMed:22528486), and cooperates with NR1I3/CAR to induce CYP2B6 (PubMed:18332083). It also modulates inflammatory responses by regulating IL1B signaling (PubMed:24379400, PubMed:25512613). Related references: PMID:18701499 PMID:22528486 PMID:18332083 PMID:24379400 PMID:25512613
• Beyond transcription, ATF5 acts as a cofactor for CEBPB to promote adipocyte differentiation (PubMed:24216764) and forms a 9-foci symmetrical ring around the mother centriole to regulate centrosome function (PubMed:26213385). Related references: PMID:24216764 PMID:26213385
• ATF5 is widely expressed with higher levels in liver, and localizes to the cytoplasm, nucleus, and centrosome. It is subject to acetylation and phosphorylation, and is involved in key cellular processes as a DNA-binding activator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 101-282 of human ATF5, covering a region likely involved in DNA-binding and transactivation. For IF/ICC, consider optimizing fixation and permeabilization to maintain epitope accessibility.
• Validated for WB, IHC-P, IF/ICC, and ELISA. In WB, a band near 31 kDa is expected; confirm specificity using positive and negative controls.
• Cross-reactivity with mouse and rat is reported. Sequence alignment across species should be verified for the epitope region to ensure consistent detection.
• For IHC-P, antigen retrieval and antibody dilution may require optimization based on tissue type and fixation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-ATF5 antibody supports studies in transcription regulation, neurobiology, and cancer research. Explore our one-stop supply chain for optimized monoclonal/polyclonal antibodies and technical consulting from concept to clinic.