Anti-ATF4 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P18848

Core Product Specifications and Parameters

Biological Background: ATF4 Function and Localization

• ATF4, also known as CREB-2 or TaxREB67, is a member of the ATF/CREB transcription factor family and plays pivotal roles in cellular stress adaptation and normal metabolic processes.
• Serves as the master transcription factor of the integrated stress response (ISR), becoming translationally induced upon EIF2S1 phosphorylation during ER stress, amino acid deprivation, mitochondrial stress, or oxidative stress. Related references: PMID:31023583, PMID:32132707
• Regulates metabolic homeostasis by activating genes involved in amino acid synthesis (e.g., ASNS), redox balance, and mitochondrial function, and can either promote cell survival or, when associated with DDIT3/CHOP, trigger apoptosis. Related references: PMID:11960987, PMID:18940792, PMID:15775988
• Involved in bone development by enhancing osteoblast differentiation and type I collagen synthesis, partly through phosphorylation by RPS6KA3/RSK2. Related references: PMID:15109498
• Exhibits widespread subcellular localization, primarily in the nucleus but also detected in the cytoplasm, cell membrane, and centrosome.
• Subject to various post-translational modifications, including phosphorylation, acetylation, hydroxylation, and ubiquitination, which modulate its activity and stability.
• Contributes to circadian rhythm regulation by periodically activating PER2 and SLC6A4 expression.
• Interacts with DISC1 in neurons to regulate synaptic plasticity and long-term memory; also binds the HTLV-I Tax-responsive enhancer element. Related references: PMID:31444471

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 260–351) of human ATF4, suggesting the antibody likely recognizes an epitope in this domain; consider sequence alignment when evaluating cross-reactivity with other species.
• ATF4 expression is highly induced under stress conditions; for low-abundance detection, pre-treat cells with ER stress inducers (e.g., tunicamycin or thapsigargin) or amino acid deprivation medium to enhance signal.
• This polyclonal antibody may detect ATF4 at ~39 kDa; note that ATF4 can undergo rapid degradation, so include protease inhibitors and avoid freeze/thaw cycles of lysates.
• For immunohistochemistry or immunofluorescence, nuclear and cytoplasmic staining patterns may be observed; validate fluorescence intensity in stress-treated vs. untreated samples.
• As a rabbit polyclonal antibody, lot-to-lot consistency and optimal working dilutions should be empirically determined for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers a reliable sourcing channel for IVD-grade antibodies, including validated polyclonal and monoclonal antibodies targeting key stress-response proteins like ATF4. In addition to catalog antibodies, we provide custom bulk reagent supply, ancillary biochemicals, and targeted raw-material procurement services—streamlining your development pipeline from concept to clinic.