Anti-ATF4 Polyclonal Antibody for WB, IF/ICC, ELISA - P18848

Core Product Specifications and Parameters

Biological Background: ATF4 Function and Localization

• ATF4 is a cyclic AMP-dependent transcription factor belonging to the CREB/ATF family, also known as CREB-2, TAXREB67, and TXREB. It binds the cAMP response element (CRE) as a homodimer or heterodimer.
• Acts as the master transcription factor of the integrated stress response (ISR), induced via alternative translation re-initiation upon eIF2α phosphorylation during ER stress, amino acid starvation, or oxidative stress. Related references: PMID:31023583 PMID:32132707
• Promotes cell recovery by activating stress-responsive genes, but when associated with DDIT3/CHOP, mediates programmed cell death by inducing pro-apoptotic genes such as TRIB3 and BBC3/PUMA. Related references: PMID:15775988
• Subcellular localization includes nucleus, nuclear speckles, cytoplasm, cell membrane, and centrosome, reflecting its dynamic shuttling and diverse regulatory roles.
• Under normal conditions, ATF4 regulates metabolic processes, osteoblast differentiation, fetal liver hematopoiesis, and synaptic plasticity; phosphorylation by RPS6KA3/RSK2 enhances osteoblast-specific gene expression. Related references: PMID:15109498
• Plays a role in circadian rhythm by binding CRE elements in PER2 and SLC6A4 promoters in a time-dependent manner.
• During HTLV-I infection, ATF4 binds the Tax-responsive enhancer element in the viral long terminal repeat, facilitating viral gene expression.

Experimental Guidance and Technical Tips

• The immunogen covers nearly full-length ATF4 (aa 1–351), offering broad epitope coverage; expect a specific band at ~39 kDa in WB.
• For IF/ICC, optimize fixation and permeabilization to detect both nuclear and cytoplasmic pools; stress induction may enhance signal.
• In ELISA, validate using recombinant ATF4 protein or cell lysates under stress conditions (e.g., tunicamycin treatment) to confirm reactivity.
• Consider using appropriate positive and negative controls (e.g., HEK293T ± ER stress) to verify specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For ATF4 and stress-response targets, we offer tailored sourcing solutions to support your assay development from concept to clinical application. Contact us to explore custom bulk orders or rare-target procurement.