Anti-ATF3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P18847

Core Product Specifications and Parameters

Biological Background: ATF3 Function and Localization

• ATF3 (Cyclic AMP-dependent transcription factor ATF-3, also known as Activating transcription factor 3) is a member of the ATF/CREB family of transcription factors.
• It binds the cAMP response element (CRE) consensus sequence 5'-GTGACGT[AC][AG]-3', present in many viral and cellular promoters, and functions as a transcriptional repressor of promoters with ATF sites, possibly by stabilizing inhibitory cofactors.
• Alternatively, ATF3 can activate transcription by sequestering inhibitory cofactors away from promoters.
• A stress-induced isoform of ATF3 exists that counteracts the transcriptional repression activity of isoform 1.
• ATF3 is localized to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications and keywords include: phosphoprotein, Ubl conjugation, isopeptide bond, and alternative splicing.
• The calculated molecular weight is ~20.6 kDa (observed ~21 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ATF3 protein (aa 1-181), suggesting recognition of multiple epitopes, which may provide robust detection across applications.
• For Western blotting, consider using whole cell lysates from cells treated with stress inducers (e.g., anisomycin, UV) as ATF3 expression is stress-inducible.
• In immunohistochemistry (IHC-P), tissue sections from stress-treated animal models or disease states may show enhanced ATF3 nuclear staining.
• For ELISA, using recombinant ATF3 protein as a standard may help in assay optimization.
• Since the antibody cross-reacts with mouse, it may be suitable for studies in mouse models; however, validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This ATF3 antibody supports transcription and cancer research applications, and our platform streamlines procurement from discovery to clinical development.