Anti-ATF3 Rabbit Polyclonal Antibody for WB, ELISA - P18847

Core Product Specifications and Parameters

Biological Background: ATF3 Function and Localization

• ATF3 is a member of the ATF/CREB family of transcription factors and binds the cAMP response element (CRE) consensus sequence 5'-GTGACGT[AC][AG]-3', which is present in many viral and cellular promoters.
• It functions as both a transcriptional repressor and activator: repression occurs by stabilizing inhibitory cofactors at promoters with ATF sites, while activation likely involves sequestering inhibitory cofactors away from promoters.
• A stress-induced isoform of ATF3 exists, which counteracts the transcriptional repression mediated by the full-length isoform (isoform 1).
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), which may modulate its activity and stability.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against a recombinant protein covering amino acids 1-181 of human ATF3; consider verifying its reactivity with relevant isoforms in your experimental system.
• For Western blot, a band around 21 kDa is expected under reducing conditions; use appropriate molecular weight markers and a loading control.
• The reported cross-reactivity with mouse and rat suggests utility in rodent models, but independent validation in the specific tissue or cell line is recommended.
• For ELISA, optimize the coating antigen and antibody concentration based on the detection range and sensitivity required for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription and stress response research, we offer validated antibody pairs, optimized polyclonal antibodies like anti-ATF3, and bulk ancillary reagents. Our sourcing capabilities also extend to rare-target raw materials, supporting your assay development and scale-up needs efficiently.